The invention discloses magnesium acetate tetrahydrate, a process for preparing the same and application of the magnesium acetate tetrahydrate. The process includes steps of synthesis, reaction, pressure filtration, concentration, re-concentration, crystallization, centrifugal discharging, drying and packaging. The magnesium acetate tetrahydrate, the process and the application have the advantages that concentration is divided into two portions, accordingly, energy can be saved, the process is high in efficiency and is simple, procedures are easy to control, raw materials for the magnesium acetate tetrahydrate are low in cost and are easily available, and the magnesium acetate tetrahydrate is low in production cost; the magnesium acetate tetrahydrate prepared by the aid of the process is a white crystal in appearance and is stable in quality; a molecular formula of the magnesium acetate tetrahydrate is C4H14O8Mg, the molecular weight of the magnesium acetate tetrahydrate is 214.46, the density of the magnesium acetate tetrahydrate is 0.8 g / l, and the melting point of the magnesium acetate tetrahydrate is 72-80 DEG C; the content of the magnesium acetate tetrahydrate is higher than 98%, the content of chlorine is 320-350 ppm, and a pH (potential of hydrogen) value of the magnesium acetate tetrahydrate is stable and keeps ranging from 7 to 9; the magnesium acetate tetrahydrate can be used for feed additives, cosmetics, catalysts and organic synthesis intermediates, can be applied to preparing magnesium uranyl acetate for measuring sodium and can be applied to the aspects of deodorization, sterilization, preservatives, textile dyeing, eosin staining, nigrosine immobilizing and the like.