The invention discloses a bactericidal flame-retardant smoke-proof moisture-proof non-toxic environmentally-friendly sound-insulating material and a preparation method thereof, the bactericidal flame-retardant smoke-proof moisture-proof non-toxic environmentally-friendly sound-insulating material comprises the following raw materials by weight: 8-12 parts of a smoke-proof flame-retardant additive,3-5 parts of a coupling agent, 3 to 5 parts of a bactericidal additive, 8 to 10 parts of modified fiber, 15 to 25 parts of a sound insulation additive, 3 to 5 of white carbon black, 12 to 14 parts ofa modified filler, 1 to 2 parts of nano zinc oxide, 1 to 3 nano titanium dioxide, 20 to 30 parts of an ethylene acrylic acid copolymer, 2 to 4 parts of a polyether polyol, 4 to 6 parts of a polyurethane emulsion, 0.7 to 1.1 parts of an antioxidant, 1 to 2 parts of a stabilizer, and 1 to 2 parts of a lubricant. The bactericidal flame-retardant smoke-proof moisture-proof non-toxic environmentally-friendly sound-insulating material has good sound insulation performance and excellent mechanical performance, has obvious flame-retarding and smoke-suppressing effect, excellent sterilization and mildew resistance, good moisture proofness and weather resistance, and no volatile harmful substances, and is non-toxic, environmentally-friendly and non-polluting; the cost of the preparation method is low, the raw materials are easy to obtain, and the bactericidal flame-retardant smoke-proof moisture-proof non-toxic environmentally-friendly sound-insulating material has high practical value and goodapplication prospects.