The invention discloses laying hen feed and preparation method of the laying hen feed. The laying hen feed comprises the following components: 4-6 parts of petasites japonicas, 3-5 parts of typha latifolia, 2-4 parts of kyllinga brevifolia, 4-6 parts of petasites japonicas, 45-55 parts of corn, 3-5 parts of spinach, 2-4 parts of mountain ramie root, 2-5 parts of coptis chinensis, 1-3 parts of euphorbia thymifolia, 3-5 parts of Chinese fevervine, 2-4 parts of centella asiatica, 1-3 parts of polygonum chinense head, 2-4 parts of mountain ramie root, 2-4 parts of garlic, 1-3 parts of leaf mustard root, 5-7 parts of bamboo leaf extract, 2-4 parts of spiral seaweed, 18-22 parts of soya bean meal, 2-4 parts of astragalus complanatus, 1-3 parts of gymnadenia conopsea, 4-6 parts of vivipara, 4-6 parts of pumpkin and 2-4 parts of carrot. The laying hen feed has the advantages that no any pigment is contained; various petasites japonicas and crops are used main raw materials, so that the nutrient ingredients are rich, the cost is low, and the taste is palatable; simultaneously, Chinese herbs such as mountain ramie root, coptis chinensis, euphorbia thymifolia, Chinese fevervine, centella asiatica, polygonum chinense head and garlic are added, so that function of removing endotoxin is realized; and typha latifolia, kyllinga brevifolia, astragalus complanatus and gymnadenia conopsea are added, so that the nutrient ingredients are rich, and further, the nutritional requirement and laying rate of laying hens can be ensured.