The invention discloses an organic fiber toughened inorganic composite artificial stone panel and a preparation method thereof. The panel comprises a surface layer, an intermediate organic fiber toughened layer, and a base toughened layer, wherein the surface layer comprises the following raw material components in parts by weight: 40-70 parts of quartz sand, 10-30 parts of a quartz powder, 20-45parts of an inorganic active powder, 0.5-4 parts of a pigment, 0.3-1 part of a water reducing agent, and 3-10 parts of water; the intermediate organic fiber toughened layer comprises the following rawmaterial components in parts by weight: 40-60 parts of the inorganic active powder, 45-65 parts of sand, 0.8-1.5 parts of the water reducing agent, 6-14 parts of water, and 4-8 parts of organic fibers; and the base toughened layer comprises the following raw material components in parts by weight: 30-50 parts of the inorganic active powder, 30-55 parts of the quartz sand, 15-20 parts of the quartz powder, 0.5-1.2 parts of the water reducing agent, 4-8 parts of water, 0.8-2.5 parts of a toughening agent. The panel has good volume stability, low shrinkage, no warping and deformation during use,extremely low water absorption, good durability, no problems such as aging, good adhesion between layers, and good ductility and no instant brittle fracture, can be used as a bearing structure, has good surface dirt resistance, and is suitable for popularization and use.