The invention discloses a
ruminant trans-
fatty acid preparation method, which comprises: (1) carrying out a
saponification reaction on butter and a
sodium hydroxide ethanol solution under a 85-90 DEG C water bath condition, adding a
sulfuric acid solution, stirring under a 45-50 DEG C water bath condition for 20-30 min, dissolving, standing, and taking the upper layer oily matter to obtain butter free
fatty acid; (2) adding
urea into absolute ethyl
alcohol according to a certain proportion, carrying out rotary
reflux at 70-85 DEG C, then adding a certain amount of butter free
fatty acid, carrying out
reflux at 77 DEG C, and carrying out inclusion at-5 DEG C; performing vacuum suction
filtration to obtain filtrate, performing rotary
evaporation on the filtrate at 65 DEG C, removing
ethanol, dissolving with deionized water at 60 DEG C, extracting upper oily matter,
drying with
anhydrous sodium sulfate, and filtering to obtain the
ruminant trans-fatty acid product. The preparation method of the
ruminant trans-fatty acid is simple in process, high in
operability, low in production cost and
energy consumption and easy to realize large-scale
continuous production.