127 results about "In vitro cytotoxicity" patented technology

Retrocyclin peptides are small antimicrobial agents with potent activity against bacteria and viruses. The peptides are nonhemolytic, and exhibit minimal in vitro cytotoxicity. A pharmaceutical composition comprising retrocyclin as an active agent is administered therapeutically to a patient suffering from a bacterial and / or viral infection, or to an individual facing exposure to a bacterial and / or viral infection, especially one caused by the HIV-1 retrovirus or other sexually-transmitted pathogens.

This invention provides an aqueous / t-butanol solvent-system, facile reconstitute, submicron-reconsitiute preliposome-lyophilaye and method of its preparation and use.In one embodiment this entails a modified method for the preparation of a submicron and stable liposome formulation of the non-cross-resistant anthracycline Annamycin is described. The optimal lipid composition was DMPC:DMPG at a 7:3 molar ratio and the optimal lipid:drug weight ratio 50:1. The selected formulation is a preliposome lyophilized powder that contains the phospholipids, Annamycin, and 1.7 mg Tween 20 per mg of Annamycin. The liposome suspension is obtained on the day of use by adding normal saline at 37° C. (1 ml per mg Annamycin) and hand-shaking for one minute. The presence of Tween 20 is essential in shortening the reconstitution step (from >2 hours to 1 minute), avoiding the early formation of free drug crystals, and reducing the median particle size (from 1.5 μm to 0.15-0.20 μm) without destruction of the liposome vesicles. The chemical stability of the preliposome powder at room temperature was >3 months and the chemical and physical stability of the liposome suspension at room temperature >24 hours. The in vitro cytotoxicity of the formulation was equivalent to that prepared by the standard evaporation method. The results of the study indicate that small amounts of surfactant may be used to enhance the reconstitution step and reduce the liposome size of lyophilized liposome formulations of lipophilic drugs.

A method of obtaining expanded and activated natural killer (NK) cells with the phenotype CD3−CD56+ and NK-like T cells with the phenotype CD3+CD56+ comprises providing a cell sample of peripheral blood from a tumor bearing subject; isolating cells from the blood sample and re-suspending the cells in growth medium; adding the isolated cells to a closed cell culture bag bioreactor at a concentration of about 0.5×106 to about 2×106 / ml of growth medium; incubating and expanding the cells of step ii) with rocking motion agitation and heating until at least 50% of the expanded cell population comprises activated NK cells and NK-like T cells; and harvesting the expanded cell suspension of therapeutically active NK-cells and NK-like T cells from the bioreactor, wherein the cells exhibit an increased cytotoxicity compared to freshly isolated cells as determined by an in vitro cytotoxicity test.

We tested the in vitro and in vivo efficacy of a recombinant bispecific immunotoxin that recognizes both EGFRwt and tumor-specific EGFRvIII receptors. A single chain antibody was cloned from a hybridoma and fused to toxin, carrying a C-terminal peptide which increases retention within cells. The binding affinity and specificity of the recombinant bispecific immunotoxin for the EGFRwt and the EGFRvIII proteins was measured. In vitro cytotoxicity was measured. In vivo activity of the recombinant bispecific immunotoxin was evaluated in subcutaneous models and compared to that of an established monospecific immunotoxin. In our preclinical studies, the bispecific recombinant immunotoxin, exhibited significant potential for treating brain tumors.

The invention discloses the application of RVX-208 as an HIV-1 latent infection reversing agent, belongs to the field of medicine, and relates to the application of RVX-208 (Apabetalone, RVX-000222) as an HIV-1 latent infection reversing agent. An important reason why HIV‑1 is difficult to be completely eliminated in the body is that HIV‑1 can lurk in the resting memory CD4 + in T cells. The RVX-208 of the present invention has good activity of activating HIV-1 latent cell pool in vitro, especially can efficiently activate the latent virus pool in HIV-infected patients, and can activate latent infection reversal agents such as protein kinase C with other mechanisms of action Drugs, histone deacetylase inhibitors and cytokines have a good synergistic effect. Compared with the known BET inhibitor JQ1, its in vitro cytotoxicity is greatly reduced, and the drug is safe and well tolerated. Therefore, RVX‑208 is expected to become a new and efficient HIV‑1 latent infection activator, so as to achieve the goal of completely eradicating the HIV‑1 latent infection pool and realizing the "functional" cure of HIV.

The invention discloses a cis-dammine dichloroplatinum (CDDP) prodrug, a preparation method and application. The structural formula of the CDDP prodrug is shown as formula (I), and is generated by theesterification reaction of activated dihydroxy cisplatin with hydrophobic molecules. Characterization of the nano-preparation by dynamic light scattering and transmission electron microscopy indicates that the nanoparticles involved in the invention are uniformly distributed and are at about 30nm. In vitro cytotoxicity experiments show that the nano-drug can significantly inhibit the proliferation of tumor cells (A549 and LoVo). In vivo experiments show that compared with CDDP injections, on the basis of reducing the systemic toxicity, the nano-drug has the effect of inhibiting the non-smallcell lung cancer A549 subcutaneous tumor, and has good market prospects and clinical application value.

The present invention discloses a method for high throughput screening (HTS) for identifying an analyte with a measurable effect on cells. The aforementioned method comprises steps of: (a) providing an array comprising a plurality of cell samples; (b) providing at least one analyte to be tested; (c) contacting said cell samples with said analyte; and (d) detecting a signal indicative of said measurable effect on cells wherein alteration of said signal over time measured on said cell sample relative to a control sample is indicative of said measurable effect of said analyte on said cell sample.The current invention further discloses means and methods for identifying an analyte selected from the group consisting of: cannabis extract or a fraction thereof cannabinoid- type constitute non cannabinoid-type constitute and any combination thereof. The analyte is indicative of cytotoxic or anti proliferative or anti mitotic or cell growth inhibitory activity in vitro.

Retrocyclin peptides are small antimicrobial agents with potent activity against bacteria and viruses. The peptides are nonhemolytic, and exhibit minimal in vitro cytotoxicity. A pharmaceutical composition comprising retrocyclin as an active agent is administered therapeutically to a patient suffering from a bacterial and / or viral infection, or to an individual facing exposure to a bacterial and / or viral infection, especially one caused by the HIV-1 retrovirus or other sexually-transmitted pathogens.

The invention is related to a nucleic acid molecule comprising a polynucleotide encoding a modified filovirus glycoprotein (GP) having at least one amino acid change located in a relatively conserved region of said GP that decreases in vitro cytotoxicity and retains immunogenicity when compared to in vitro cytotoxicity and immunogenicity of a wild type filovirus GP, and related modified filovirus GPs, plasmid DNAs, recombinant viruses, adenoviruses, pharmaceutical compositions, vaccine compositions, antibodies that are specifically reactive with the modified filovirus GPs, and related methods of making and using the same.

The invention relates to a novel staurosporine analogue and a preparation method and applications thereof, wherein two staurosporine analogue molecules with a novel structure is prepared from the Streptomyces Sp.FMA which is separated from the soil sample of the Sanya Mangrove in Hainan Island of China and has the strain preservation number of CCTCC M 2010021. The in vitro cytotoxicity test proves that the compound has cytotoxicity to leukemia cell HL-60 and lung cancer cell A549 and can be used as the cell antiblastic.

The invention discloses a method for testing in vitro cytotoxicity of cigarette smoke. The method is characterized by comprising the following steps of: 1) preparing a laboratory reagent; 2) performing cell inoculation culture; 3) performing cigarette smoke contamination; 4) dyeing with water-soluble tetrazolium-1 (WST-1); and 5) obtaining a result and analyzing. Compared with the prior art, the method is characterized in that a test step for washing cells for multiple times is eliminated in the whole test process, so that the method is easy and convenient to operate; a step of replacing a nutrient solution is not required during dyeing with the WST-1, and a diluted nutrient solution can be directly added for reacting; formazan produced by the WST-1 is water-soluble, so that a subsequent dissolution step is eliminated; and absorbance detection can be finished in 2 hours after WST-1 dyeing, so that the test period is shortened, and the method is quick in comparison with the conventional testing method. The measuring method is quick and convenient to operate, and also has the advantages of high sensitivity and stable result; and the method can be applied to smoke cytotoxicity test of multiple cell lines.

The invention provides a 3D printing preparation method of a skin tissue engineering scaffold and an in vitro cell toxicity testing method of the scaffold. The 3D printing preparation method comprisesthe following steps: preparation of double aldehyde nano-crystalline cellulose used for a scaffold material, preparation of a gelatin solution, preparation of a double aldehyde nano-crystalline cellulose and gelatin compound hydrogel, and printing of the 3D tissue engineering scaffold. The problem that the tissue engineering scaffold needs the requirements of high porosity and high precision is solved by using a 3D biological printing technology. DAC is taken as a cross-linking agent, cross-linking occurs with GEL through a Schiff base reaction to form a network structure, so that the 3D printing tissue engineering scaffold has excellent mechanical property and is unlikely to crack, and simultaneously the additional value of plant fiber is also improved. The DAC / GEL hydrogel has good biocompatibility, is free of toxic and / or side effects or immunologic rejection, has biological activity besides degradation characteristic, and is extremely beneficial for the growth and differentiationof cells and the implementation of cell functions.

The invention discloses an application of a pit-hole composite micro-nano-structure polysaccharide micro-sphere to preparation of haemostatic wound dressing. According to GB / T16886.5-2003 in-vitro cytotoxicity judgment standards, acquired pit-hole composite micro-nano-structure polysaccharide micro-sphere extracting solution has 0-grade cytotoxicity under the concentration of 10-40 micrograms / L and shows good biocompatibility, bleeding can be effectively stopped within 20s-50s, good physical and chemical property is achieved, hemolysis ratio is smaller than 5%, national standards are met, themicro-sphere has an instant haemostatic function for a liver injury wound surface (haemostatic time is shorter than 5 seconds), haemostatic materials are completely degraded 72 hours after, and any residue is omitted. The pit-hole composite micro-nano-structure polysaccharide micro-sphere can stop bleeding within 20 seconds when being used for excessive bleeding of a femoral artery, surgical woundsurface recovers normal one week after, and any haemostatic micro-sphere sample residues are omitted.

The invention discloses a spraying method for a water-based two-component wood paint for teenage furniture. The method belongs to the production field of furniture. The method comprises the following steps: taking TB-003 water-based two-component wood paint prime coating and TB-032 water-based two-component wood paint finishing coating as raw materials, and carrying out pre-treatment on the materials of the furniture, polishing for the surface of the furniture, primary roller coating for the prime coating, drying in an ultraviolet-light drying tunnel, polishing for the prime coating, secondary roller coating for the prime coating, high-finish-degree sanding and polishing for the prime coating, spraying for the finishing coating, and ultraviolet-light drying to prepare the finished product. The product is the furniture for teenagers, and has the following main advantages: 1. the product has an odour removal function, the surface of a paint film, the interior of a cabinet body and a store environment are free from the odour of the paint, and the odour generated by wood can be effectively sealed by the paint; 2. the paint film is high in hardness, resistant to chemicals, water, pollution and after-tack, high in transparency, and good in hand feeling; and 3. the coating layer of the paint film is high in resistance comprising resistance to allergy, irritative reaction to the skin, and in-vitro cytotoxicity.

The invention belongs to the technical field of biomedicine production or the fields of protein polypeptide drugs and biomedical engineering, and in particular relates to a polypeptide which has high affinity with an integrin receptor and an application of the polypeptide like cancer diagnosis and treatment. The polypeptide is composed of five amino acids, and the sequence is arginine-tryptophan-arginine-asparagine-methionine (Arg-Trp-Arg-Asn-Met). On the basis of an in-vitro flow cytometry affinity determination experiment, the polypeptide disclosed by the invention has quite strong targeting on high-expression cells of the integrin receptor, and through an in-vitro laser confocal cell uptake experiment, the polypeptide can be targeted to high-expression tumor cells of the integrin receptor alpha-v-beta-3 and can basically avoid targeting on low-expression tumor cells and normal cells of the integrin receptor alpha-v-beta-3. An in-vitro cytotoxicity result shows that the 5-peptide disclosed by the invention is basically free from toxicity on cells, and the 5-peptide has a good application prospect in the diagnosis and the treatment of cancers.

The present invention relates to an ortho-naphthoquinone derivative, a preparation method and medical application thereof, and provides a compound with the structure shown as a formula I, a preparation method and application thereof in the manufacture of tumor medicaments. The compound of the invention has a hybrid structure of ortho -naphthoquinone with thiadiazole [3,2-a] pyrimidine, strong anti-cancer activity, metabolic stability and good selectivity. In vitro cytotoxicity and topoisomerase I inhibition tests show that the compound has strong inhibition on tested cancer cells and topoisomerase I; and NQO1 activity test shows that the compound is an effective substrate for NQO1, and mediated by NQO1, the compound cycles by a redox reaction, produces a large amount of reactive oxygen to induce oxidative stress, and selectively kills tumor cells. The compound can be used as an anti-cancer drug or a lead compound for further development. The method of the present invention has the characteristics of greenness, environmental protection, easily available raw materials, simple operation, and high yield.

The invention discloses a method for testing in vitro cytotoxicity of cigarette smoke. The method is characterized by comprising the following steps of: 1) preparing a laboratory reagent; 2) performing cell inoculation culture; 3) performing cigarette smoke contamination; 4) dyeing with water-soluble tetrazolium-1 (WST-1); and 5) obtaining a result and analyzing. Compared with the prior art, the method is characterized in that a test step for washing cells for multiple times is eliminated in the whole test process, so that the method is easy and convenient to operate; a step of replacing a nutrient solution is not required during dyeing with the WST-1, and a diluted nutrient solution can be directly added for reacting; formazan produced by the WST-1 is water-soluble, so that a subsequent dissolution step is eliminated; and absorbance detection can be finished in 2 hours after WST-1 dyeing, so that the test period is shortened, and the method is quick in comparison with the conventional testing method. The measuring method is quick and convenient to operate, and also has the advantages of high sensitivity and stable result; and the method can be applied to smoke cytotoxicity test of multiple cell lines.

The invention belongs to the pharmaceutical field of traditional Chinese medicines and relates to a prenylflavanone compound shown formula 1 and a new use thereof in preparation of anti-tumor medicaments. The flavonoid compound is extracted from a Sophora tonkinensis ethyl acetate part and proved to have stronger inhibition effect for tumor cell lines of lung cancer, prostatic cancer, nasopharyngeal cancer or colorectal cancer through an in vitro cytotoxicity screening test, wherein the compound comprises 5.41-7.19 mu g / mL of GI50 which has the inhibition effect on the cells of the nasopharyngeal cancer, 6.48-7.21 mu g / mL of the GI50 which has the inhibition effect on the cells of the lung cancer, 5.10-5.15 mu g / mL of the GI50 which has the inhibition effect on the cells of the prostatic cancer and 7.52-14.81 mu g / mL of the GI50 which has the inhibition effect on the cells of the colorectal cancer. The compound can be further used for preparing the anti-tumor medicaments for clinical treatment of the lung cancer, the prostatic cancer, the nasopharyngeal cancer or the colorectal cancer.