A straw organic decomposition agent is prepared from bacillus subtilis, brewer's yeast and aspergillus oryzae, the content of bacillus subtilis is larger than or equal to 15 billion cfu / g, the content of the brewer's yeast is larger than or equal to 20 billion cfu / g, the content of aspergillus oryzae is larger than or equal to 500 million cfu / g, and the number of effective living bacteria in the decomposition agent is larger than or equal to 265 million per gram. In the straw organic decomposition agent, only three original strains exist, bacteria, fungus living bacteria and an enzymic preparation are compounded, multiple components achieve a synergistic effect in the decomposition process, a cellulase preparation is cultured by mixing bacillus subtilis, brewer's yeast, aspergillus oryzae and the like, multiple kinds of cellulase systems are generated, good applicability is achieved, lignin ingredients in straw can be damaged fast, cellulose and hemicellulose in organic materials are exposed, and the decomposition time of the straw is shortened. A method for screening strains is efficient and practical, the production and fermentation process is simple, the production period is short, the number of the living bacteria and the enzyme activity level are high and stable, the use effect of the prepared decomposition agent is superior to that of an existing product, and wide industrialization prospects are achieved.