The invention relates to the technical field of biological
fermentation, in particular to a method for identifying a dominant
metabolic pathway of VFA degradation in AD based on an energy efficiency ratio. The method comprises the following steps: determining boundary conditions of a VFA
metabolic pathway; and acquiring data in the operation process of the AD reactor. According to the method for identifying the dominant
metabolic pathway of VFA degradation in AD based on the energy efficiency ratio, process parameters such as gas components, VFA concentration, pH value,
bicarbonate ion concentration and the like in AD (
anaerobic digestion) are regularly detected, a thermodynamic function in a
reaction system is corrected according to a metabolic
chemical equation of VFA in combination with
phase state, temperature and substrate and product concentration factors, and the dominant metabolic pathway of VFA degradation in AD is identified. Calculating a
delta G value corresponding to each metabolic pathway; the
electron transfer mole number (Ne) involved in each metabolic reaction is calculated, and
delta G is normalized into a form (
delta G / Ne) of
electron transfer per mole; preliminary identification of the dominant metabolic pathway can be realized through the physicochemical parameters easy to obtain in the AD
system, and the method has the remarkable advantages of high efficiency, convenience and high universality.