The invention discloses a collagen polypeptide peeling mask which is mainly prepared from the following raw materials in parts by weight: 2-5 parts of hydroxyethyl cellulose, 3-7 parts of arbutin, 1-1.5 parts of sodium alga acid, 4-5 parts of polyethylene glycol, 3.5-4.0 parts of fish skin collagen polypeptide, 6.3-7.0 parts of trehalose, 0.15-0.3 part of grape seed extract, 2-4 parts of licorice root extract, 0.1-0.2 part of phycocyanin, 0.05-0.15 part of antioxidant, 0.5-1.5 parts of preservative, 0.3-0.6 part of compound antibacterial agent, 3-7 parts of glycerinum, 1-3 parts of water-soluble azone and 100-120 parts of natural hydrosol or flower water. The preparation method comprises the following steps: obtaining an intermediate product solution A; obtaining an intermediate product solution B on the basis of the intermediate product solution A; obtaining an intermediate product solution C on the basis of the intermediate product solution B; and finally, adding the residual raw materials into the intermediate product solution C, pouring the obtained materials into a mold for molding, drying at the temperature of 50 DEG C, thereby obtaining the collagen polypeptide peeling mask. The invention also discloses a preparation method of the collagen polypeptide peeling mask.