85 results about "Megascale engineering" patented technology

The invention discloses a star quantum key distribution network without a trusted relay based on a MDIQKD protocol. The star quantum key distribution network disclosed by the invention is expanded into a star network structure based on a point-to-point MDIQKD system; therefore, network resource consumption is saved; and the difficulty due to network expansion can be solved. According to a light source structure provided by the invention, equipment used in a time feedback scheme, a phase feedback scheme and a polarization feedback scheme is simple and reusable; therefore, the quantum key distribution network is relatively simple, relatively easy to integrate and low in cost; furthermore, feedback requirements can also be reduced through further optimization; and thus, the foundation is laid for further realizing safety, high-speed, large-scale engineering treatment and practicability of quantum secret communication.

The present invention relates to an engineering method for producing biochar soil from dredging sediment, and belongs to the field of solid waste resource utilization. The biochar soil is prepared by uniformly mixing the raw materials of dredging sediments, poultry manure and dry straw according to a certain weight proportion, adjusting the C / N ratio and moisture content of the mixture, adding a certain proportion of a nitrogen retention and water-retaining agent; carrying out windrow composting by a mechanical turning and static forced ventilation combined manner for about 30 days, maintaining the pile at 55-65 DEG C for 10-12 days until the full maturity of the compost products. The biochar soil is utilized according to actual need. The invention realizes large-scale engineering treatment of dredging sediment, poultry manure and crops straws, provides an ecological way to solve the non-point source pollution caused by dredging sediment and poultry manure, and air problem caused by crops straws burning; and the compost products can be used for ecological engineering construction, so as to organically combine environmental benefits, economic benefits and ecological benefits.

The invention discloses a method for degrading organic pollutants in water through electrochemical cathodic activation of persulfate, and relates to a method for degrading the organic pollutant in water, aiming at solving the problems of existence of iron sludge, damage to an electrode, residue of bivalent iron ions and complexity in operation caused by addition of an iron salt existing in the prior art. The method comprises the following steps: injecting water containing the organic pollutants to be treated into a cathode / anode electrode pair reactor; adding the persulfate; and then performing processing under the conditions of certain persulfate concentration and certain cathode electrode voltage to finish the water treatment on the organic pollution to be treated. The method has the advantages that: 1, the persulfate is activated without adding any chemical agent, so that the problems of secondary pollution, large sludge and the like can be avoided; and 2, the method is easy to operate, low in energy consumption and can finish activation of the persulfate under the conditions of smaller energy input, and the method can be generalized and used in large-scale engineering. The method is mainly used for removing the organic pollutants in the water.

The invention provides a safe disposal method for a high-concentration arsenic residue. According to the method, a waste residue containing arsenic is crushed and screened at first so as to improve full contact between a chemical added in the process of later treatment and the waste residue containing arsenic; oxidation treatment is carried out to allow As<3+> in an environment to be oxidized into As<5+>; a desorption agent is added to promote desorption of AsO4<3->, AsO3<3-> and AsO2<-> in the environment and entry of the AsO4<3->, AsO3<3-> and AsO2<-> into an environmental solute; a stabilization agent is added in the process of stabilization to allow free AsO4<3->, AsO3<3-> and AsO2<-> in the environment and elements in the stabilization agent to form stable precipitates; then a pH value is adjusted to ensure environmental conditions for existence of the stable precipitates; a curing agent is added in the process of curing to further limit relocation diffusion capability of pollutants in the environment; and oxidation and shaping are carried out to ensure long-term security of the residue. The safe disposal method provided by the invention can realize safe disposal of a waste residue containing high-concentration arsenic (less than 1.0*10<5> mg / kg) and has the characteristics of a good restoration effect, high stability, a short restoration period, a low fund level, simple operation, great treating capacity, engineerability on a large scale, etc.

The invention relates to a preparation method of oxalate by CO gas phase coupling synthesis using a regular catalyst. In the method, a fixed bed reactor is adopted, a catalyst bed consists of a regular structural catalyst loaded with noble metal; CO and vaporized nitrite are introduced into a reactor for reacting on the regular catalyst so as to generate the oxalate at the conditions that the pressure is 0.1-2MPa, the reaction temperature is 80-200 DEG C, and N2 is taken as a carrier gas, wherein the volume ratio of the materials is as follows: N2:CO:nitrite is 20-80:5-60:10-40, and the retention time is 0.5-10 hours. The method of the invention improves mass transfer efficiency of the reaction materials between the gas phase and the solid phase or among the gas phase, the liquid phase and the solid phase, increases the contact area of the reaction materials and the catalyst and reduces the usage amount of the catalyst in the noble metal, wherein the usage amount of the catalyst noblemetal Pd is saved by more than 86%. The method of the invention has the characteristics of high catalyst activity, low cost, convenient and fast replacement and the like, and is beneficial for realizing large-scale engineered application.

The invention discloses a modular multilevel converter system and a control system and control method of the modular multilevel converter system. An upper computer control layer comprises an operation personnel monitoring system, wherein the operation personnel monitoring system is connected with a system control unit. A system-level control layer comprises the system control unit, wherein the system control unit connected with a valve control unit. A valve-level control layer comprises the valve control unit, wherein the valve control unit issues sub-modular trigger pulse signals to sub-modular controllers. A modular-level control layer comprises the sub-modular controllers, wherein the sub-modular controllers are arranged in sub-modulars of a modular multilevel converter valve in a dispersed mode, and each sub-modular controller is used for having control over connection and disconnection between an upper power modular and a lower power modular of the corresponding sub-modular. Integrated control over a whole modular multilevel converter is achieved at different control priority levels by adopting a layered structure with a plurality of control units according to the requirement of the modular multilevel converter for special control, and the good foundation is laid for large-scale engineering application of the modular multilevel converter.

The invention discloses a multi-core parallel simulated annealing method based on a thread constructing module, which mainly solves the problem of the operating efficiency of a simulated annealing algorithm in large-scale engineering application and the problem of the utilization of computer multi-core resources. The method uses the advantage that the thread constructing module supports multi-core processor parallel algorithm and supports the expanded thread nesting parallel, and introduces a group optimization mechanism construction parallel algorithm of various groups on the basis of the former serial simulated annealing algorithm. The method comprises the following steps: firstly, setting environmental variables; secondly, constructing a parallel module; thirdly, setting initial parameters; fourthly, performing independent optimization on each initial state; fifthly, acquiring the current optimal state and the current optimal result; sixthly, executing temperature reducing process; and finally, obtaining the optimal state and the optimal result. The multi-core parallel simulated annealing method has simple and flexible processes, is easy for expansion, accords with the trend that computers are developed to have multi-processors and multi-core architectures, and is a convenient and quick parallel simulated annealing design method with strong practicability.

The invention discloses an application of molasses in chromium-polluted soil remediation and a remediation method. The molasses are used for remediation of the chromium-polluted soil. The remediation method comprises the specific steps of: pretreating the chromium-polluted soil, preparing molasses agent, determining the added amount of the molasses agent, mixing the polluted soil and the molasses agent, reacting the polluted soil and the molasses agent, conserving the remediated soil and detecting the concentration of chromium pollutants in the soil at the later stage to ensure the stability. The application and the remediation method disclosed by the invention have the advantages that the molasses are used as a biochemical reduction agent in the chromium-polluted soil remediation, and the remediation efficiency is high; due to addition of the molasses, an organic carbon source and trace elements are provided for native-bacteria type microorganisms in the soil, the breeding metabolism of the microorganisms is promoted, and the biological remediation action is enhanced; in addition, the source of the molasses is wide, the price is low, no negative and positive ions are introduced, and the soil structure after remediation is improved, so that the improved soil is beneficial to following development and utilization, can be used for large-scale engineering implementation and can effectively prevent secondary pollution.

The invention discloses an Ethernet passive optical network (EPON) system with a hand-in-hand protection structure. The EPON system with the hand-in-hand protection structure comprises two optical line terminals, a light distribution network and a plurality of optical network units. Each optical line terminal comprises at least a pair of passive optical network (PON) ports, the light distribution network comprises at least a three-fiber optical caple, three optical fibers are all connected with a plurality of light branching units, the first optical fiber is connected with a PON port of the first optical line terminal, the second optical fiber is connected with a PON port of the second optical line terminal, the third optical fiber is connected between the other PON port of the first optical line terminal and the other PON port of the second optical line terminal, and the middle portion of the third optical fiber is cut off. The optical network unit is respectively connected to the two optical line terminals through a pair of light branching units to form the hand-in-hand structure, one light branching unit of the pair of light branching units is placed on the first optical fiber or on the second optical fiber, and the other light branching unit of the pair of light branching units is placed on the third optical fiber. The EPON system with the hand-in-hand protection structure can be used in large-scale engineering, so that infrastructure cost can be saved, and the protection effect of an original optical path can be kept.

The invention discloses an acoustic control method of an active structure of a sealed cavity. The method comprises the following steps that firstly, the acoustic control problem of the structure of the sealed cavity is resolved; secondly, a local control problem solution scheme is provided; thirdly, coordination between controller bodies is carried out. According to the method, the bodies can be conveniently added or removed, and the rest of a system do not need to be redesigned, and it is indicated that a surface system has high expandability. In addition, the method has the self-governing performance, flexibility and sociality, and dynamic noise in the sealed cavity can be effectively treated. In other words, the method has the advantages of being capable of meeting intelligence, strong robustness and expandability needed by large-scale engineering application and the like.

The invention discloses a floating pendulum type ocean manual downwelling device utilizing wave energy and a control method. The device is based on the lever principle, by converting the wave force borne by a floating floater into a pressure head, surface layer oxygen-enriched water can be injected into bottom layer water, the oxygen shortage condition is relieved, and the water quality is improved. The device has a wave self-adaptive control system, the self mechanical structure can be changed according to the magnitude of the wave force, and therefore the optimal downwelling flow can be generated, and the wave energy can be efficiently utilized. In addition, in the environment that the wave condition is severe, the control system makes the whole device in the standby state, the overload protection effect is achieved, and the device is made to have good survival capacity. Because a device power system is mainly composed of mechanical components, the device is convenient to maintain and install. In conclusion, large-scale engineering application of the device in home and abroad oxygen-shortage sea areas rich in wave energy resource is hopeful, and the condition of large-area water oxygen shortage is relieved.

The invention provides a high-temperature-resistant and anticorrosive composite ceramic coating which is a high-technology environment-friendly energy-saving coating resistant to high temperature and acid-base corrosion and high in abrasion resistance. An advanced high-temperature-resistant silicon-fluorine modified film-forming solution is adopted, various kinds of pigment are formed in a chelating mode under the closed high-temperature condition, and the resistant temperature can be 1200 DEG C. As compact ceramic glaze is formed at high temperature, the coating has the advantages of being high in hardness, resistant to impact, long-acting in corrosion, good in adhesive force, resistant to abrasion, long in service life and the like, meets the requirement for large-scale engineering construction in industry in China, and can be widely applied to the acid-base long-term corrosion environment, the organic solvent corrosion environment, chemical material storing tanks, and the high-corrosion and high-friction environment.

The invention provides a maintenance-free completely automatic filter machine, consisting of at least two groups of filter units; each group of filter units comprises a plurality of filter cores which are arranged inside the sealed shell of the filter machine; the water inlet pipeline of the filter machine is provided with a water inlet pipeline pressure divider; each formed branch pipe is connected with the internal hole at the lower end of each filter core corresponding to the filter unit; each branch pipe is provided with an electronic valve; a tailing outlet is arranged below each group of filter units; the tailing outlet is connected with the internal hole at the lower end of each filter core of the filter unit; and the tailing outlet (10) is provided with an electronic valve. The filter machine breaks through the shortage of the connection type of the existing filter core of the filter machine, achieves the object of limitless irregular arrangement of the filter cores, can achieve the backwashing function by the self-pressure difference, completes the backwashing function without effecting the normal do-work process, creates the precondition for the continuous operation of the filter machine, and provides solid foundation for fine filtration of large-scale engineering.

The invention provides an all optical wavelength converter and all optical wavelength conversion method for a dual-semiconductor optical amplifier structure and relates to a hybrid wavelength division time division multiplex passive optical network system. A downlink signal light can be divided into two paths through a 1:2 optical splitter, i.e., one path of light is compensated by an erbium-doped fiber amplifier (EDFA), combined by a wavelength division multiplexer and then sent to a user terminal; the other path of light successively passes through the EDFA, a first semiconductor optical amplifier and an adjustable optical attenuator and then is used for the probe light input of a cross-gain modulation structure; after being divided by the wavelength division multiplexer, an uplink signal light is successively connected with the EDFA, the adjustable optical attenuator and a polarization controller and is used for the pump light input of the cross-gain modulation structure; and the probe light and the pump light can be input into an optical coupler, converted through a second semiconductor optical amplifier and a band pass filter and then output. The all optical wavelength converter and the all optical wavelength conversion method are in accordance with the optical passive and colorless requirements; and meanwhile, the all optical wavelength converter has a simple system structure, thus reducing the operation and maintenance costs of the system and being beneficial to the application and later maintenance for large-scale projects.

The invention belongs to the environmental protection industry and the field of three-waste environmental protection purification, and relates to a method for closed-circuit microorganism desulfurization and elemental sulfur recovery, particularly to a process for biological desulfurization treatment of sulfur-containing industrial gases (such as biogas, natural gas, coal chemical industry processgas, refinery gas, sulfur-containing industrial waste gas, and the like, wherein the purposes of closed-circuit biological desulfurization, maximized recovery of elemental sulfur, standard emission of waste gas and near-zero emission of waste liquids can be achieved through chemical absorption, biological oxidation, elemental sulfur recovery, membrane separation, anaerobic reduction and other processes. According to the present invention, the treatment method can meet the biological desulfurization requirements, can solve the difficult problem of elemental sulfur production through sulfide oxidation and enhanced separation, and can be used for biogas, natural gas, coal-to-gas, refinery gas, sulfur-containing industrial waste gas and other industrial large-scale engineering.

The invention discloses a method for restoring ecology integrating substrates, hydrology and organisms in a bank collapse lakeside. The method comprises the steps of: firstly, carrying out necessary slop cutting on high banks or abrupt slopes according to the slop condition of a lakeshore line, and enlarging the depth of plants for protecting the lakeshore to the maximum; secondly, selecting different types of plants for restoration according to the fluctuation rule of the water level in a lake reservoir and different range areas of the lakeshore line; cultivating different types of or mixed matching of plants, such as arbor, shrub and grasses, for restoration in places above the highest water level, and cultivating emergent aquatic plants with a strong root system and high fixation strength for restoration in places below the highest water level; and thirdly, for the restoration of places below the highest water level by using the emergent aquatic plants, selecting a drought period before and after the spring; carrying out tunneling operations by using a hydraulic dredge; arranging two wave-damping dams along the lakeshore line; using a shallow water area near the inner side of the dam as a restoration growing area of the emergent aquatic plants; and restoring the emergent aquatic plants by subculturing the roots and the stems. The invention provides a technical method which can be carried out in a large-scale engineering manner by integrating substrate restoration, hydrology restoration and organism restoration, and the natural ecological structures and the landscapes of the lakeside are restored to the maximum.

The invention discloses a keyword optimization classification method based on a fuzzy genetic algorithm, comprising the steps of: (1) randomly assorting units to be optimized into each island, and initializing the N units to be different units in each island; (2) selecting the units which simultaneously participate the internal genetic operation of the island and transfer to other islands; (3) crossing the units selected by the step (2); (4) performing nonuniform mutation operation to all units in each island; (5) calculating assessed values for all units in each island; (6) searching local area for the units in each island; and (7) selecting the units in each island, and judging whether the assessed value satisfies optimization rules and then judging whether repeating the abovementioned steps. According to the method, the problem of solving large-scale engineering can be solved, the intelligence is high, the optimization algorithm is clear and simple, and the optimization accuracy is high.

The invention discloses a novel soil purifying agent and application thereof to polybrominated diphenyl ethers (PBDEs) contaminated soil restoration. As PBDEs has a strong hydrophobicity and is easy to adsorb intensively by soil, and very low in bio-availability, the treatment of the PBDEs contaminated soil is very difficult. To solve the problem, an anionic surfactant and iron group compound which are environment-friendly, cheap and easy to get are utilized to form a metal complex, namely the soil purifying agent, according to the molar ratio of (1:3)-(1:5); after being uniformly mixed with the soil, the soil purifying agent is added with humic acid, and soaked by water, then the soil purifying agent can be used for efficiently and rapidly purifying contaminated soil while the soil property is not changed and the bio-availability is improved. The novel soil purifying agent is suitable for PBDEs contaminated soil restoration, and has an excellent application prospect on the aspect of large-scale engineering application.

The invention provides a method for calculating a three-dimensional position of a ground point based on a sequence satellite-borne SAR image. The method comprises following steps of S1, extracting image coordinates of any two SAR images of a ground point, and calculating a satellite three-dimensional position and the slope distance between the ground point and the satellite according to the imagecoordinates; S2, calculating satellite base line parameters formed by any two SAR images according to the satellite three-dimensional position; S3, acquiring satellite speed corresponding to the two SAR images, and calculating object space conversion parameters according to the satellite three-dimensional position and the satellite speed; and S4, calculating the three-dimensional position of the ground point according to the object space conversion parameters and the object space conversion parameters. According to the invention, the three-dimensional position of the ground point is calculatedin a directly displayed manner by applying the platform parameters and the system parameters of the satellite-borne SAR remote sensing image without an initial approximate value of the ground point;there is no any iteration and approximation process in the calculation; the calculation efficiency is improved; and large-scale engineering application is facilitated.

The invention relates to a reverse-flow type horizontal flow hydrogen substrate bio-membrane reactor based on carbon dioxide as a carbon source. The reactor consists of a horizontal rectangular reactor box (5), a water inlet (2), a reflux water inlet (1), an overflow water outlet (9), a reflux water outlet (8), a sludge discharge opening (7), six interfaces (11) with inner threads on a box cover and six membrane assemblies providing H2 and CO2, wherein every two membrane assemblies are one group, third guide plates (6), second guide plates (4) and first guide plates (3) are sequentially arranged among every group of membrane assemblies and between the membrane assemblies and the water inlet (2); and the bottom of the first guide plate (3) is fixedly connected with the inner bottom wall of the reactor box, a channel is reserved in one side of the first guide plate (3), the bottoms of the other two guide plates are provided with channels, two sides are symmetrical and are fixedly connected with the front and back side walls of the box in a staggered manner so as to form symmetrical channels. The reactor disclosed by the invention is good in treatment effect, convenient to operate and maintain and low in cost, and easily treats oxidized contaminants in underground water on a large scale through engineering.

The present invention discloses a testing method and device for simulating deformation characteristics of a soil mass under dynamic train load; by modifying a traditional one-dimensional static consolidometer, it is possible for the device to apply axial dynamic cyclic load, it is convenient to develop quantitative analysis for axial compressive deformation of soil mass under dynamic train load and lateral confinement, equivalent dynamic compression curve and empirical dynamic consolidation equation for the soil mass are obtained, equivalent dynamic deformation parameters for the soil mass are further calculated, and the demand for large-scale engineering practice is met for through the experiment with multiple groups of samples. Compared with traditional geotechnical engineering indoor dynamic testing methods using dynamic triaxial instruments and hollow cylinder torsional shear apparatus, the testing method and device disclosed herein have simple structure and reasonable production cost, the testing method is quick to perform, the result data are highly stable, and basis is laid for the accurate calculation and design of railway subgrade dynamic long-term settlements.

The invention discloses a sewage treatment method using micro-aerobic autotroph to remove nitrogen. The method comprises the following steps of 1. carrying out micro-aerobic oxidizing on organic matters in sewage, and meanwhile realizing partially synchronous nitrification, denitrification and nitrosification; 2. strictly controlling dissolved oxygen, proportionally converting ammonia nitrogen into nitrite nitrogen, and making the ratio of the ammonia nitrogen to the nitrite nitrogen in the discharged sewage approach to 1; and 3. enriching the anaerobic ammonia oxidation bacteria, and ensuring that the ammonia nitrogen and the total nitrogen in the discharged sewage reach standards. The method has the advantages that the existing urban sewage with low carbon-nitrogen ratio and the sewage with high ammonia nitrogen ratio can be treated highly efficiently, the technology is simple, the operation is convenient, the method is suitable for large-scale engineering, and the good economical, social and environmental benefits are realized.

The invention relates to a method for acquiring the electric field distribution of a large-sized complex aircraft by using a fast boundary element method, which is technically characterized by solving the problem of the fast computation and analysis of a large-sized electrostatic field of the aircraft in the field of aircraft design by adopting the boundary element method. The method comprises the following steps of: dividing a medium interface into smooth curved surfaces with parametric equations by adopting a Nessler discretization method, and projecting integral points to the medium interface to form boundary integral points through the parametric equations; and constructing a wavelet transform matrix on the basis of the multiscale grouping of the integral points, and carrying out matrix compression, computation and storage on a boundary element coefficient matrix. The invention overcomes the shortages of complex computation, large computation amount and low precision of the coefficient matrix in the traditional wavelet boundary element method and effectively reduces the internal storage occupying amount and the computation time of boundary element analysis, thereby solving theproblems of a large-scale project with millions of freedom degrees; and in addition, the invention can be applied to the electric field computation and analysis of the large-sized complex aircraft inan aerospace field and electric field analysis in other project fields.

The invention discloses a construction engineering integrated management system. The system comprises an integrated processor which is used for core data processing operation, a power supply which is used for transmitting power to the whole system, a memory which is used for storing integrated data, an on-site management module which is used for on-site coordination management of a construction site, and a project management module which is used for engineering project schedule control. The integrated processor is connected with the power supply, the on-site management module, the data transmission module, the memory and the project management module. According to the invention, the construction engineering integrated management system integrates attendance management, GPS positioning, on-site management and other functions into a whole body, which is advantageous for centralized or separate management of construction site admission personnel in large-scale engineering construction; each admission person can be timely notified in case of an emergency; and the information scheduling of the entire project is greatly improved.

The invention belongs to the technical field of environment engineering and particularly relates to a method for separating and recycling heavy metal from garbage incineration fly ash and conducting hazard-free treatment on the fly ash. The method comprises the steps that after the household garbage incineration fly ash is diluted through an acidic sulphur oxidation bacteria culture medium solution, sulphur balls are added, so that acidic leach liquor containing the heavy metal is obtained; after a proper amount of alkali is added for pH adjustment, hydrogen sulfide gas is introduced into theacidic leach liquor; the hydrogen sulfide gas reacts with the heavy metal, so that sulfide precipitation is formed; and after solid-liquid separation is completed, recycling is conducted in the form of the sulfide precipitation, so that the pure heavy metal is obtained. The treatment process is implemented at the normal temperature and normal pressure; energy consumption is low; the process is simple; the large-scale engineering potential is achieved; a biological leaching energy source substance, namely sulphur, is recycled to be reused, so that the post-acidification problem of the treated fly ash is solved; the heavy metal entering the leach liquor is subjected to reduction and precipitation conducted through sulfate, so that the heavy metal is separated or recycled or removed effectively; and the sulfate and pH problems are solved at the same time.

The invention discloses a characterization method for hardness of electrode materials under the combined action of electrochemistry and substrate effects. The method includes the steps that a charging and discharging test is conducted on the electrode materials of a lithium ion battery to obtain the electrode materials in different charging and discharging states, and an indentation experiment is conducted on the electrode materials through an indentation tester to obtain an indentation load displacement curve, so that the composite hardness between the electrode materials and a substrate is extracted under a certain charging and discharging state, and finally the composite hardness is substituted into a constructed analytic theoretical model between the hardness and the indentation size to obtain the hardness of the electrode materials. The hardness of the electrode materials of the lithium ion battery in different charging and discharging states can be successfully extracted by using the analytic theoretical model obtained through the method, and the characterization method is convenient, fast and beneficial for large-scale engineering application.

The invention provides a method of calculating three-dimensional positions of ground points by using sequential satellite borne remote sensing images. The method comprises the following steps: first,extracting two-dimensional image coordinates of the ground points on each satellite borne remote sensing image and calculating the three-dimensional auxiliary coordinates corresponding to the ground points on each satellite borne remote sensing image by using satellite sensitive quaternion parameters corresponding to each satellite borne remote sensing image; second, calculating the transformationcoefficient formed by any two satellite borne remote sensing images according to the satellite position corresponding to each satellite borne remote sensing image and the three-dimensional auxiliarycoordinates corresponding to the ground points; and finally, calculating the three-dimensional positions of the ground points by using the transformation coefficient, the satellite positions and the ground point auxiliary coordinates. The method directly calculates the three-dimensional positions of the ground points explicitly by applying platform parameters and camera parameters of the satelliteborne remote sensing images without initial approximate values of the ground points. Iterative and approximate processes are avoided in calculation, so that the calculating process is simplified effectively, and the method is more suitable for scaled engineering application.

The invention provides an expression type pre-T-vector for quickly cloning and expressing the product PCR of the target gene, and a preparation and an application thereof. The advantages of the expression type pre-T-vector mainly lie as follows: the target gene can be cloned and expressed by one step method, thereby omitting complicated recombination processes; the product PCR is not needed to be purified and enzyme cut in construction process, and linearized T-vector can be obtained by the method that the pre-T-vector is single enzyme cut by XcmI with high enzyme cutting efficiency without using other restrictively restriction enzymes; the target gene is not needed to be induced by expensive IPTG, which can ferment engineering bacteria in large scale with lower cost; and the sequencing can be identified after the target gene is expressed, and then transformants whose expression results are up to expectation are sent to the DNA sequencing company to be sequenced, so the sequencing success rate can be improved, and the expenses can be saved.

The present invention discloses a preparation method for an anatase type bentonite-based porous TiO2 nanometer material. The preparation method is that the organic bentonite after dehydration and drying or the organic bentonite abandoned after wastewater treatment is added with the mixture of amine and titanate or only added with titanate; then after well mixing and centrifuging, the supernate is abandoned and the solid part left will be removed from surfactant and organic materials through drying and high-temperature calcinations, thus the porous TiO2 nanometer material is obtained. The material prepared by the invention is of large specific surface area, smaller grain size, single particle size and uniform pore distribution, wherein, the aperture ranges between micropore and mesopore and the pores are in high sequence degree. The material is mainly anatase type and is provided with relatively high catalytic activity, which can be applied to the indoor organic pollutant purification field. At the same time, the present invention provides a very good solution to the ultimate disposition of abandoned organobentonite, and makes good use of the abandoned organobentonite after wastewater treatment to achieve the treatment of a waste with another waste. The preparation method is of low energy consumption, simple preparation process, optimal pH control and no need of acid, which is suitable for large-scale engineering applications.