The invention discloses a new cultivation method of paulownia seedlings. The method includes following steps: S1, selecting, proportioning and treating a nutritional matrix; S2, collecting seed roots;S3, burying the roots; S4, cultivating, hardening seedlings, and transplanting, wherein compound nutrients include nitrogen, phosphorus and potassium elements, trace elements and compound amino acid,the nitrogen element is urea, the phosphorus element is potassium dihydrogen phosphate, the potassium element is potassium sulfate, the trace elements include calcium, magnesium, zinc, copper and iron which are added in a soluble compound form, and the compound amino acid is composed of alanine, arginine, cystine, glycine, lysine and proline. A standardized cultivation greenhouse and a standardized treatment process are adopted, so that a farming method of field cultivation is broken through, occupied area is reduced, lesion probability of the paulownia seedlings is lowered, seedling cultureperiod is shortened, and yield is increased.