The invention discloses a high-calcium gristle beef board and a preparation process thereof. The high-calcium gristle beef board takes beef and pork gristle as main raw materials; a certain amount ofsoybean protein, edible salt, white granulated sugar, konjaku flour, a food additive, edible spice, a compound emulsifier and water are used as auxiliary materials; the beef board is obtained throughthe steps of beef slicing, beef acid discharging, injection material water making, injection, primary rolling of beef, secondary rolling of beef, ultrasonic treatment, gristle beef board forming, vacuum quick freezing, demolding, packaging, boxing and warehousing in sequence, is suitable for hotpots, barbecues and the like, has the advantages of being rich in nutrition and calcium, and enriches the types of traditional beef products.