The invention discloses thermosetting high temperature resisting printing ink for a touch screen as well as a preparation method of the printing ink. The formula of the printing ink comprises the following raw materials in parts by weight: 55-65 parts of modified organic silicon resin, 20-30 parts of titanium dioxide, 5-15 parts of powder packing, 3-8 parts of a diluent, 0.5-1.4 parts of a dispersant, 0.1-0.3 part of a leveling agent, 0.05-0.1 part of a defoamer, 1-2 parts of a silane coupling agent and 2-5 parts of a curing agent. The preparation method comprises the following steps: mixing the modified organic silicon resin, a pigment, the powder packing, 0.35-0.95 part of the dispersant, 0.05-0.15 part of the leveling agent and 2-5.4 parts of the diluent to obtain a main agent; and adding the curing agent, the defoamer, the silane coupling agent as well as remaining leveling agent, dispersant and diluent into the main agent, and dispersing, filtering and discharging. The printing ink coating is high temperature- resisting in coating, excellent in color retention at a high temperature of 300 DEG C, good in adhesion to a base material, high in hardness, resistant to acetone wiping and resistant to ITO (Indium Tin Oxides) etching liquids.