The invention provides a biological repair membrane and a preparation method thereof. In the prior art, the existing biological repair membrane has the following disadvantages that the mechanical strength is poor, the suture point has the cerebrospinal fluid leakage risk, the tissue regeneration and repair promoting performance is weakened, the residual toxicity exists after the degradation, the biocompatibility is poor, and the adhesion between the repair material and the brain tissue is easily caused, and other complications are easily induced. According to the present invention, the animal-derived membrane tissue is subjected to cell removing and antigen removing, and a natural polymer material coating capable of being adsorbed and providing bleeding stopping effect is coated on the treated animal-derived membrane tissue so as to solve the problems in the prior art; the method specifically comprises: pretreatment, degreasing with an organic reagent, cell removing and antigen removing with an alkali liquid, coating preparation, vacuum compression and freeze-drying, packaging, and sterilization so as to complete the preparation of the biological repair membrane; and the biological repair membrane has advantages of bleeding stopping, antibacterial property, adhesion, natural three-dimensional space structure retaining, good mechanical property, good biocompatibility, good tissue growth induction, degradation non-toxicity, wide raw material source, low price, and easy mass-production.