The invention discloses NV-series environment-friendly type rubber floor glue stock, which is composed of the following raw materials (by weight): NV rubber, zinc oxide, active magnesium oxide, stearic acid, an anti-ageing agent, titanium dioxide, argil, a fire retardant HP800, aluminium hydroxide, zinc borate, a fire retardant BN-18C, white carbon black, nano-kaolin, ultrafine calcium carbonate, an antistatic agent, a dispersant, a flow promoter, an anti-ultraviolet agent, an antibacterial agent, a deodorant, a vulcanizing agent S-80, a promoter, a vulcanizing agent CLD-80 and a pigment. The invention also discloses a preparation method of the NV-series environment-friendly type rubber floor glue stock. The problem that existing flooring is not water-resistant, wear-resistant, waterproof or anti-microbial, is expensive in price and releases harmful gas or radioactive element is solved in the invention.