The invention relates to the field of seed storage and packaging, and provides a manufacturing method of an antibacterial packaging material for seed storage, and the antibacterial packaging material comprises an antimony-doped tin oxide nanoparticle material and an indium-doped tin oxide nanoparticle material; the manufacturing method of the antibacterial packaging material comprises the following manufacturing steps: S1, preparing an antimony-doped tin oxide nanoparticle material; s2, preparing an indium-doped tin oxide nano particle material; s3, film blowing: three-layer co-extrusion is adopted, a film body is made of a PE material, and antimony-doped tin oxide and indium-doped tin oxide nanoparticles are added into the plastic film body; growth of mould around seeds can be remarkably inhibited, and the antibacterial time is long. Meanwhile, the used materials are low in raw material cost, simple in process, easy to put into industrial production and non-toxic, and use of nano-silver antibacterial materials with potential safety risks and very high cost is avoided.