The invention discloses a quick-acting biological pesticide aqueous solution. The quick-acting biological pesticide aqueous solution comprises the following substances in parts by weight: 5 to 19 parts of polyhedrosis virus, 20 to 28 parts of bergamot oil, 5 to 8 parts of ethylicin, 11 to 14 parts of flavonoid, 35 to 61 parts of matrine, 11 to 16 parts of verticillium chlamydosporium, 6 to 9 parts of iuyangmycin powder, 20 to 30 parts of stem oil, 3 to 7 parts of propiconazole, 5 to 16 parts of diammonium phosphate, 4 to 14 parts of Tween 80, 25 to 34 parts of hirsutella thompsonii fisher powder, 35 to 40 parts of choline chloride, and 80 to 95 parts of water. The quick-acting biological pesticide aqueous solution has a good effect, a quick-acting property, and a plurality of effects of adjusting crop growth, killing pests, dispelling pests, killing bacteria and resisting viruses, and is particularly safe and environment-friendly, and high in quick-acting efficacy by taking water as a solvent.