The invention provides a magnetic nickel-cobalt oxide supported gold catalyst, a preparation method and application thereof, and a preparation method of 2, 5-furandicarboxylic acid, and belongs to the technical field of catalysts. The magnetic nickel-cobalt oxide supported gold catalyst provided by the invention comprises a magnetic nickel-cobalt oxide carrier and gold nanoparticles supported on the magnetic nickel-cobalt oxide carrier, wherein the chemical composition of the magnetic nickel-cobalt oxide carrier is CoNixOy, x ranges from 1 to 5, and y is the number of oxygen atoms meeting the requirement that the valence state of CoNixOy is zero. The magnetic nickel-cobalt oxide supported gold catalyst provided by the invention has high catalytic activity on preparation of 2, 5-furandicarboxylic acid by oxidation of 5-hydroxymethylfurfural, has high selectivity on a target product 2, 5-furandicarboxylic acid, has good stability, can realize separation of the catalyst and the product through a simple and convenient physical magnetic separation mode, and the catalyst is high in repeated utilization rate and is an efficient, specific, green and environment-friendly catalyst.