3 results about "Reagent standard" patented technology

The present application relates to the technical field of biometrics and in vitro diagnosis standardization, and particularly relates to a preparation and value determination method of alpha-synuclein solution standard substance, comprising the following steps: cloning a gene coding alpha-synuclein into a prokaryotic expression vector for recombinant expression, purifying the protein raw material with purity greater than or equal to 99% through affinity chromatography and ion exchange chromatography; diluting and sub-packaging the protein raw material for storage to obtain the solution standard substance; determining the value of the standard substance by using an isotope dilution mass spectrometry method based on amino acid analysis, calculating the mass concentration value by measuring the peak area ratio of stable amino acids and isotope-labeled amino acids after hydrolysis, and tracing the value to the international unit system through the amino acid primary standard substance; and performing uniformity testing and stability investigation on the standard substance, solving the problem of lack of standard substance with metrological traceability in the alpha-synuclein detection field, and obtaining the standard substance which can be used for value transmission and detection result standardization among different in vitro diagnosis platforms.

The invention discloses a kit for improving detection specificity of latex immunoturbidimetry, and belongs to the technical field of immunodetection. The invention provides a composite buffer system for improving the detection specificity of latex immunoturbidimetry. The composite buffer system comprises the following components in working concentration: 5-50g / L of a sealing agent, 0.1-5mL / L of a nonionic surfactant and a specific interferent blocking agent. The invention further provides a high-specificity detection kit for the protein markers in body fluid or blood. The high-specificity detection kit comprises a reagent 1, a reagent 2, a standard substance and the composite buffer system. According to the invention, various components with different action mechanisms are creatively and scientifically matched to form a synergistic systematic solution, so that the problems of poor detection specificity and accuracy of latex immunoturbidimetry in the prior art are solved.

The technical scheme of the present application provides a continuous monitoring device and a continuous monitoring method, which can improve the more accurate measurement of the low concentration of the target in the sample. The continuous monitoring device is used for continuously monitoring the concentration of the target in the external environment, and the continuous monitoring device comprises a sampling part, a liquid storage tank, a container, a detector and a processor. The sampling part collects the sample from the external environment, and the liquid storage tank stores the standard reagent containing the target with the first concentration. The container is in communication with the sampling part and the liquid storage tank, and a first specified amount of sample and a second specified amount of standard reagent are mixed to obtain a test reagent. The detector is in communication with the container, and detects the intensity signal reflecting the concentration of the target in the test reagent. The processor determines the concentration of the target in the sample according to the intensity signal.