The invention provides lanthanum aluminate-doped fuel cell electrolyte and a preparation method. The preparation method comprises the following steps: mixing hydrated nitrate of lanthanum, hydrated nitrate of aluminum, a doping element A, a doping element B, acetylacetone, isopropyl alcohol and a methylcellulose aqueous solution to prepare a La1-xAxAlyB1-yO3 type compound precursor, coating the surface of a substrate layer with the La1-xAxAlyB1-yO3 type compound precursor to form a thin film precursor, and performing high-temperature sintering to prepare a cubic perovskite type doped lanthanumaluminate thin film, namely obtaining the fuel cell electrolyte. According to the method provided by the invention, an octahedral interstice inside a crystal is enlarged; an interstice conduction channel similar to an apatite structure is formed inside a crystal lattice, and the number of oxygen vacancies is increased by doping, so that the transmission efficiency of electrolyte oxygen ions is effectively improved; the thermodynamic stability is high, and the working temperature is relatively low; meanwhile, the preparation process is simple; the used raw materials are low in cost, so that the anthanum aluminate-doped fuel cell electrolyte is low in energy consumption and low in preparation cost and has a relatively good economical advantage.