34 results about "Protein need" patented technology

A gadus dried meat floss cake comprises, by weight, 180-190 parts of wheat flour, 30-40 parts of lotus root starch, 40-50 parts of rice flour, 10-20 parts of black soybean flour, 20-30 parts of konjaku flour, 8-10 parts of yeast powder, 50-60 parts of egg, 40-50 parts of codfish, 10-20 parts of eel meat, 50-60 parts of dried beef floss, 4-5 parts of calculus bovis, 6-7 parts of pearl, 5-6 parts of albizzia flower, 4-5 parts of radices hemerocallis, 5-6 parts of marigold, 4-5 parts of konjak leaves, 40-50 parts of yellow rice or millet wine, 20-30 parts of white vinegar, 10-20 parts of fresh ginger slice, 10-60 parts of white granulated sugar and appropriate water. According to the gadus dried meat floss cake, a plurality of kinds of powders such as the konjaku flour and the lotus root starch are used as the cake pig trotter materials, nutrition is abundant, the flavor is good, gadus and other meat containing animal protein are added, and protein needed by the human body can be supplemented. Meanwhile, albizzia flower and other extracts of Chinese herbal medicines with the function of soothing the nerves are especially added, and therefore the gadus dried meat floss cake has the very good function of soothing the nerves and is suitable for most people.

The invention discloses an ecologically balanced and nutritional adult turtle feed formula. The mass ratios of the raw materials are as follows: 41-50 parts of white fishmeal, 19-30 parts of red fishmeal, 3-5 parts of corn protein powder, 1-3 parts of fermented soybean meal, 1-4 parts of wheat gluten powder, 2-4 parts of beer yeast, 2-5 parts of soybean protein concentrate, 15-30 parts of starch, 0.1-0.2 part of calcium dihydrogenphoshate, 2-4 parts of premix and 0.2-0.4 part of phytoxanthin; the mass ratios of the raw materials of the multidimensional premix are as follows: 5000-6000 parts of vitamin A, 2100-3200 parts of vitamin D, 85-165 parts of vitamin E, 8-17 parts of vitamin K, 19-28 parts of vitamin B1, 17-33 parts of vitamin B2, 22-35 parts of vitamin B6, 0.06-0.15 part of vitamin B12, 190-330 parts of nicotinic acid, 28-44 parts of D-pantothenic acid, 4-16 parts of folic acid, 220-260 parts of biotin and 270-360 parts of inositol. The ecologically balanced and nutritional adult turtle feed formula disclosed by the invention can be used for effectively supplementing various minerals, trace elements and protein needed in the growth process of turtles, and can be used for ensuring that the artificial feeding turtles have similar nutritional value with that of the wild turtles and ensuring the cooking taste of the artificial feeding turtles same as that of the wild turtles.

The invention discloses novel mycobacterium tuberculosis extracellular nuclease and application of the novel mycobacterium tuberculosis extracellular nuclease, and proves that Rv (Rotavirus) 0888 is extracellular nuclease of mycobacterium tuberculosis. Based on a sequence analysis, no homology exists between the Rv0888 nuclease and known extracellular nuclease, so that the Rv0888 is proved to be novel extracellular nuclease; activity exerting of Rv0888 protein needs the assistance of divalent ions, and the optimal temperature and the optimal pH (Potential of Hydrogen) of the Rv0888 protein are respective 41 DEG C and 6.5. The invention also provides application of four kinds of traditional Chinese medicine monomers of oleuropein, 6-gingerol, corylifolinin and acteoside for inhibiting the novel mycobacterium tuberculosis extracellular nuclease. Site-directed mutagenesis research shows that a catalytic action of H353 residue, D387 residue and D438 residue on the activity of the Rv0888 is achieved; an in-vivo infection experiment proves that the persistence capacity of recombined mycobacterium smegmatis Rv0888 NS/MS and Rv0888 S/MS in a lung of a mouse is obviously higher than that of pMV262/MS, obvious pathological change can be generated in the lung of the mouse by the Rv0888 NS/MS and the Rv0888 S/MS, and the Rv0888 is proved to be necessary for mycobacterium infection and pathogenicity.

The invention mainly relates to the technical field of planting and discloses a germination accelerating method for purple carrot seeds. The germination accelerating method for the purple carrot seeds comprises the steps of seed dipping, seed fermentation, seed enzymolysis and germination acceleration of the seeds. The germination accelerating method for the purple carrot seeds is simple and specifically comprises the steps that the purple carrot seeds are dipped in an edible alkali solution, so that the seed skin is rapidly softened; seed fermentation is conducted through lactobacillus acidophilus, remaining alkali is removed, the seed skin structures are destroyed, micromolecule nutritional ingredients are generated and promoted to enter the seed skin, and the activity of embryos is improved; after enzymolysis, seed skin external impurities come off, a great number of proteins needed by growth of the seeds is provided at the same time, and the germination rate is improved. In the seed germination accelerating process, through spraying of a germination accelerating agent, nutrients can be provided, insect resisting and sterilizing are achieved, the humidity is kept, the stress resistance of the embryos is improved, growth is accelerated, the germination rate reaches 93%, the germination accelerating time is shortened to 3-4 days, and the planting cost is saved by 15.7%. The components of the germination accelerating agent are simple and wide in source, the utilization ways of grape flowers are increased, and the economic income of each peasant household planting purple carrots is increased by 8.3%.

The invention discloses a perch feed formula and a preparation method thereof, and relates to the technical field of fish farming. In the perch feed formula, imported white fish meal, spray-dried blood globulin powder and antarctic krill powder and other high-quality protein raw materials are added, so that the digestion and absorption rate of perch is high, the growth rate is improved, and the protein needs are met; vitamins, probiotic, and broken yeast powder are comprehensively complemented to enhance the body constitution of perch, improve immunity, promote digestion and absorption and reduce the occurrence of enteritis; the feed formula is treated by an expansion process to effectively improve the absorption and utilization rate of a feed, an expander used is simple in structure and convenient to use, and has high expansion efficiency, and a communicating pipe of the expander can be effectively prevented from being clogged by the feed; the preparation process of the feed formula has the advantages of reducing the damage to the nutrient composition of the feed, ensuring the particle size and mixing uniformity of the feed, increasing the energy level in the feed, and having obvious economic and environmental benefits.

The invention discloses a compound feed used for polyculture of bighead silver carps and herrings and a preparation method thereof, and relates to the field of fish breeding; and the compound feed used for polyculture of the bighead silver carps and the herrings is composed of a floating feed and a sinking feed. The sinking feed is mainly used for feeding the herrings which live at middle and lower layers of the water; and various animal and plant proteins are used in cooperation so as to provide proteins needed for herring growth. The floating feed is mainly used for feeding the bighead silver carps which live at an upper layer of the water; the floating feed is relatively low in protein content, and is added with various Chinese herbal medicines; and a main purpose of the floating feed is to improve disease resistance of the bighead silver carps. The compound feed adopts different formulas for different fishes so as to avoid nutrient waste; moreover, the compound feed improves survival rate and growth rate of the fishes in polyculture mode so as to increase yield. According to the preparation method of the compound feed used for polyculture of the bighead silver carps and the herrings, differential treatments are carried out during preparation processes according to different feeding habits of the bighead silver carps and the herrings; a fermentation process is added into the preparation processes of the floating feed according to the characteristic of the bighead silver carps, namely fancy on sour rotten foods; and differential feeding of the bighead silver carps and the herrings are ensured.

The invention discloses a method for marking protein. The method comprises the following steps: selecting a protein needing to be marked, using a functional molecule with carboxyl or thiocarboxyl as a substrate, using unsaturated sulfonium salt as a carboxyl activator, and carrying out a solid-phase peptide bond generation reaction on the marked protein in an alkaline buffer solution; in a buffer solution, carrying out amine ester exchange reaction activated by unsaturated sulfonium salt and carrying out specific modification reaction on amino groups on the protein; and identifying the specific modification condition by means of western blot or mass spectrum. Functional molecules containing carboxyl or thiocarboxyl are used as substrates, unsaturated sulfonium salt is used as a carboxyl activating agent, and in different types of buffer solutions, different types of various proteins can be marked through amine ester exchange reaction. According to the method, safe and low-toxicity sulfonium salt activated ester is used as a labeling molecule, protein can be labeled efficiently and selectively, and the method can be used for activity-based protein analysis and labeling of antibody molecules in a drug-coupled antibody.

The invention provides health-care protein powder with the effect of improving the body immunity. The health-care protein powder is prepared by the following components in percentage by weight: 65-85% of soybean isolated protein, 10-15% of whey protein powder, 5-10% of egg yolk immunoglobulin powder, 1-5% of bovine colostrums immunoglubin powder, 2-4% of sesame powder, 0.5-3% of soybean lecithin, 0.5-1% of silicon dioxide, 2-4% of starch, 0.001-0.002% of bifidobacterium, 1-3% of scale extract and 0.001-0.1% of aspartame, and the sum of the percent of the components is 100%. The invention researches and develops a protein type health-care powder with the effect of improving the body immunity; by optimization of the formula and the process, the obtained protein powder is comprehensive in nutrition, not only can supplement the protein needed by a human body and maintain normal immunologic mechanism of the body, but also is low in content of fat in protein type materials, can not cause the cardiovascular disease due to excessive intake of the fat, can be used as a modern health-care food and has wide market prospect.

The invention discloses chick feed and a preparation method thereof, and belongs to the technical field of feed. The feed is prepared from, by weight, 85-95 parts of corn, 30-40 parts of wild buckwheat, 5-10 parts of zeolite powder, 10-20 parts of soybean meal, 5-15 parts of rapeseed meal, 3-8 parts of eggshells, 1-3 parts of pumpkin powder, 5-10 parts of distillers' grains, 1-3 parts of sugarcaneleaves, 2-6 parts of wood vinegar, 0.5-1.5 parts of phytase and 0.5-1.5 parts of diaminocaproic acid. Raw materials of the chick feed are simply and reasonable matched, energy and protein needed by growth of chicken can be completely met, microelements are balanced, and healthy growth of the chicken is facilitated.

The invention relates to a health drink, in particular to a health drink capable of supplying key nutrient elements such as a plurality of microelements and proteins needed by a human body and improving the health conditions of the human body. The health drink mainly aims to solve the problem that the various conventional drinks are all prepared to meet the requirement of human beings on palatability and little attention is paid to the health of consumers. Natural soy bean, which contains rich proteins and microelements and has high output and low price and is well stocked, is taken as the main raw material. The preparation method comprises the following steps of: cleaning and immersing the soy bean, sprouting the soy bean, and crushing the soy bean; adding carrot juice, pumpkin juice, longan juice, medlar juice and shaddock juice; and preparing the mixed raw juice drink according to the modern drink processing technology. The health drink has the advantages of supplying the key nutrient elements such as the plurality of microelements and proteins needed by the human body and improving the health conditions of the human body.

The invention discloses a cultivation method for soybean root nitrogen fixation pleurotus eryngii, and belongs to the technical field of fungus cultivation. A pleurotus eryngii culture medium comprises, by mass, 70-80% of soybean straw root smashing fibers, 10-20% of soybean straw smashing fibers, 5-8% of soybean dregs, 1% of lime powder and 1% of gypsum powder. The cultivation method for the soybean root nitrogen fixation pleurotus eryngii has the advantages that soybean roots have the higher nitrogen fixation capacity and can supply sufficient nitrogen sources to pleurotus eryngii strains for growth, soybean straw and the soybean dregs are added to supply lignin and protein needed by the growth of the stains, and the lime powder and the gypsum powder are beneficial for absorption and growth of the pleurotus eryngii; the pleurotus eryngii produced by the culture medium cultivating strains is good in quality, short in cycle, high in yield and good in mouthfeel, the waste soybean roots and straw are used as cultivation raw materials, the waste materials are changed into things of value, therefore, the production cost is saved, economic sustainable development is achieved, and the method has a good development prospect in the field of pleurotus eryngii cultivation.

The invention discloses a feed used for juvenile prawns. The feed comprises, by weight, 25-40 parts of fish meal, 10-20 parts of soybean meal, 10-15 parts of corn flour, 5-10 parts of wheat gluten flour, 5-15 parts of peanut bran, 3-8 parts of soyabean lecithin, 3-5 parts of yeast powder, 3-8 parts of shrimp powder, 0.1-0.5 parts of edible salt and 1-4 parts of a premix. The feed has balanced nutrition, contains various energy and proteins needed by growth of the prawns, and has the advantages of high conversion efficiency, high stability, improvement of the digestion and absorption ability of the prawns, and feed investment reduction, so the production cost is reduced, the disease prevention ability of the prawns is improved, and guaranteeing is provided for producing green and nuisance-free prawns.

The invention patent relates to the technical field of feed processing, and discloses an animal feed and a preparation method thereof. The animal feed, according to the mass percentage, comprises thefollowing components: bean cake 20-30%, straw 10-20%, cottonseed hull 5-10%, rapeseed 8-12%, purslane 3-5%, dandelions 4-6%, waste tea 1-4%, and earthworm powder 0.5-1.2%. The animal feed provided bythe invention is simple in preparation, low in cost, rich in nutrients, safe and reliable, has no toxic or side effects; raw materials of natural plants including the purslane, dandelions and waste tea have a balanced arrangement and sufficient nutrients; and the dandelions, purslane and waste tea are also rich in vitamins, mineral amino acids and other substances beneficial to animal growth, andimmunity improvement of the animals; and compared with the traditional animal feed, the feed is added with the high protein earthworm powder to ensure the protein needed for animal growth and is moreconducive to improving the quality of animal meat products.

The invention provides an attractive feed for goldfishes and a preparation method thereof. The attractive feed for the goldfishes is characterized by being composed of the following ingredients in parts by weight: 10-20 parts of fish meal, 10-20 parts of soybean meal, 3-6 parts of rapeseed meal, 6-12 parts of rice protein powder, 5-10 parts of corn, 10-20 parts of alpha-starch, 5-8 parts of kiwi fruit powder, 3-6 parts of hawthorn fruit powder, 2-5 parts of fish oil, 1-3 parts of calcium dihydrogen phosphate, 2-4 parts of a premix, 10-15 parts of shrimp pulp, 2-8 parts of DMPT, and 2-6 parts of betaine. By using a natural feed raw material, namely the shrimp pulp, as an ingredient of an attractant, the attractive feed is capable of ensuring daily supplement of proteins needed by the goldfishes, as well as attracting the goldfishes to eat by the smell; moreover, the shrimp pulp, the DMPT and the betaine are used together as attractant ingredients, so that synergistic effect can be achieved so as to enhance the attracting effects of the feed.

The invention discloses a preparation method of recombinant protein. In existing methods, some methods are high in cost and low in output, some methods are both high in technical requirements and cost, and preparing new protein needs long period, and is high in risk and poor in flexibility. The invention is characterized by cloning a recombinant protein gene into a eukaryotic expression vector and transfecting the vector into a mice ascites cell line, building a stabilized transfection transgene cell line through screening in vitro and cloning, and injecting the cell line into a mice abdominal cavity; and using the mice abdominal cavity as a transgene bioreactor for the transgene cell line to express the recombinant protein, extracting ascites after the ascites is formed and extracting and purifying the recombinant protein from the ascites. According to the method, the preparation of the recombinant protein is not only simple, highly efficient, quick and well applicable, but also low in cost, high in yield and good in natural activity of the recombinant protein.

The invention discloses a natural protein substance compound lotion. The natural protein substance compound lotion is prepared from chlorhexidine gluconate and peptidoglycan; generally, the natural protein substance compound lotion is added into water so as to be prepared into the lotion, the concentration of the chlorhexidine gluconate in the lotion is 0.1 to 1 percent g/ml, and the concentration of the peptidoglycan in the lotion is 0.05 to 1 percent g/ml. The natural protein substance compound lotion disclosed by the invention is used for restraining bacillus coli, staphylococcus aureus and tritirachium album. Generation of obvious bioactivity between the chlorhexidine gluconate and the peptidoglycan is promoted through combination of the chlorhexidine gluconate and the peptidoglycan, the excellent treatment effect is generated, peripheral blood cells can be increased while the bacteriostatic activity of the chlorhexidine gluconate is expressed, various protein needed for skin repairing can be compounded, macrophage and lymphocyte can be activated.

The invention relates to an organism identification method based on a membrane-bound complex. The membrane-bound complex at least consists of two parts including transmembrane protein and a signal molecule, and the transmembrane protein and the signal molecule are linked by a chemical bond or a flexible amino acid fragment. According to the provided rapid, simple and effective organism identification method, only an organism and the protein need to be mixed for incubation for 10 minutes, the fluorescence intensity of different organisms ranges from several thousands to several millions, the resolution among the organisms of different species is high, and the method has high repeatability and accuracy.