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results about "Cryphonectric acid" patented technology
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Polyampholyte cell cryoprotectants, methods of making and polyampholyte cell cryopreservation solutions
Pending
CN122250450A
Dead animal preservation
Polylysine
Electrolyte
The application provides a polyamphoteric
electrolyte
cell
cryoprotective agent
and a preparation method and application of a polyamphoteric
electrolyte
cell
cryopreservation
solution, relates to the fields of biomedical materials technology and
cell
cryopreservation
, and aims to solve the problems of low cell
recovery
rate and short low-temperature storage time of cells in the existing cell
cryopreservation
technology. The polyamphoteric
electrolyte
cell
cryoprotective agent
comprises
polylysine
,
polyacrylic acid
and
graphene
oxide
connected through a
chemical bond
, the
mass
ratio of the
polyacrylic acid
and the
polylysine
is 10:1-2:1, and the
mass
ratio of the total
mass
of the
polyacrylic acid
and the
polylysine
to the mass of the
graphene
oxide
is 1:2-10:1. The polyamphoteric electrolyte cell
cryoprotective agent
can not only reduce the osmotic damage of cells in the freezing and thawing process by adjusting the
osmotic pressure
, but also improve the freezing efficiency and inhibit the formation and growth of
ice crystals
. The protective agent can be used in the fields of
stem cell
cryopreservation, immune cell cryopreservation and the like due to the excellent cell protection performance and good stability.
Owner:
SUZHOU UNIV
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