3results about How to "Efficient catalytic performance" patented technology

The application belongs to the technical field of biology and particularly relates to a kind of biomimetic enzyme and its application in degrading aflatoxin.The raw material of the biomimetic enzyme includes polypeptide and divalent copper ion, the sequence of the polypeptide is shown in SEQ ID No.1, specifically: Ac-HCHLKLKLKL-CONH2, the N terminal of the polypeptide is acetylated, and the C terminal is amidated.The biomimetic enzyme is constructed by sequencing design and metal coordination to construct an active site, is stable in catalyzing AFB1 rupture in wide pH and temperature, has the advantages of good tolerance, high catalytic efficiency, and has potential application advantages in the application in grain and feed purification.

The invention discloses a preparation method of an efficient and stable industrial-grade OER nickel-iron catalyst. According to the method, nickel nitrate hexahydrate, ferric nitrate nonahydrate and anhydrous sodium nitrate are uniformly dissolved in ultrapure water to form a mixed precursor solution, and then the catalyst is prepared through an in-situ reaction by adopting a constant-temperature and normal-pressure impregnation method. According to the invention, anhydrous sodium nitrate is utilized to introduce abundant nitrate ions to carry out intercalation modification on a nickel-iron layered structure, so that on one hand, the energy barrier of oxygen evolution reaction (OER) is effectively reduced, and the kinetic process of the reaction is obviously accelerated; on the other hand, the unique interlayer anion intercalation structure can effectively anchor active sites, and dissolution and loss of active elements such as iron in the reaction process are inhibited to a great extent. The prepared catalyst is high in catalytic activity, simple and convenient in preparation process and excellent in attenuation resistance, and can meet the requirement of long-acting stable operation for hundreds of hours under industrial-grade large current density.

This invention belongs to the field of enzyme engineering technology, and relates to a sucrose synthase mutant and its application in the catalytic synthesis of uridine diphosphate glucose (UDPG). The sucrose synthase mutant of this invention is derived from *Nitrosomonas cerevisiae* (European *Nitrosomonas*). Nitrosomonas europaea Wild-type sucrose synthase (ATCC 19718) was obtained through single-point or combined mutations. The in vitro enzyme activity, UDP conversion rate, and thermostability of the sucrose synthase mutant of this invention are significantly superior to the wild type. Fusion of a soluble tag at the N-terminus of the mutant significantly enhances its soluble expression and catalytic efficiency. Under conditions of 100 mM UDP and 100 mg / L enzyme loading, the mutant of this invention achieves a UDPG yield of 72.1 mM within 60 min, with a maximum UDP conversion rate of 73.7%, an improvement of approximately 20 percentage points compared to the wild type.