4 results about "Sodium pentachlorophenate" patented technology

The invention discloses a method for measuring the residual quantity of sodium pentachlorophenate in eggs, and belongs to the technical field of detection.The method comprises the steps that edible parts of egg samples are taken and subjected to homogenization treatment, and egg liquid samples are obtained; the method comprises the following steps: adding an internal standard reagent, a protein precipitant, dilute sulphuric acid and normal hexane into an egg liquid sample, uniformly mixing, centrifuging, taking an upper organic phase, and purifying for derivation; preparing a series of pentachlorophenol standard working solutions, and adding an internal standard reagent into the pentachlorophenol standard working solutions; adding a derivatization reagent into the organic phase and the pentachlorophenol standard working solution, and carrying out derivatization treatment; putting the derived organic phase and a pentachlorophenol standard working solution into gas chromatography-tandem mass spectrometry equipment, and testing on a machine to obtain the content of pentachlorophenol in the organic phase; and determining the residual quantity of the sodium pentachlorophenate in the egg sample according to the content of the pentachlorophenate. The technical effect of improving the detection accuracy of the residual quantity of the sodium pentachlorophenate in the eggs is achieved.

The application discloses a preparation method of a molecular imprinting electrochemical sensor for detecting sodium pentachlorophenol and detection application thereof, and is characterized by comprising the following steps: after ultrasonic dispersion of a sodium pentachlorophenol molecular imprinting polymer suspension, the suspension is drop-coated on the surface of a treated glassy carbon electrode, and the molecular imprinting electrochemical sensor is obtained by standing and drying at room temperature; and a sodium pentachlorophenol detection method is also provided and comprises the following steps: the sensor is placed in a phosphate buffer solution containing Ru(bpy)3 2+ TPrA is polarized, and an initial ECL signal intensity I is recorded; after a to-be-detected PCP-Na solution is added, the ECL intensity I0 is determined by standing and reacting, the ECL signal change value ΔI=I-I0 is taken as a response index, the PCP-Na concentration in the to-be-detected PCP-Na solution is calculated according to the linear relationship between the PCP-Na solution concentration and ΔI, and the method has the advantages of good selectivity and stability, high sensitivity and accuracy.

The invention provides a sodium pentachlorophenate rapid detection kit suitable for bamboo materials and aquatic products, the rapid detection kit is a colloidal gold kit, and the colloidal gold kit comprises a reagent plate, a microporous reagent and a diluent; the diluent is a phosphate composite solution. According to the valve, rapid, efficient and sensitive detection of sodium pentachlorophenate residues in two matrixes is realized, the pretreatment process is simplified, the detection time is shortened, meanwhile, the accuracy and the stability of a detection result are ensured, and the actual requirements of field detection and batch screening are met.

The present invention discloses a pentachlorophenol sodium hapten, an artificial antigen thereof and their applications in immunoassay. The structure of the pentachlorophenol sodium hapten is shown in Formula I, and the structural formula of the pentachlorophenol sodium artificial antigen is shown in Formula II. The preparation method of the pentachlorophenol sodium hapten of the present invention is simple. The introduced arm of the designed pentachlorophenol sodium hapten not only has active groups, but also completely retains the phenol structure of the target analyte, increasing the recognition sites. The content of the product pentachlorophenol sodium hapten has been greatly improved. Moreover, the pentachlorophenol sodium artificial antigen and monoclonal antibody prepared based on the hapten of the present invention have strong detection specificity. When used in test strips, they can detect more trace amounts of pentachlorophenol sodium, with a detection sensitivity of 0.8 μg / L for pentachlorophenol sodium and higher sensitivity.